Tropical Doctor > Volume 38, Number 2 > Pp. 97-99

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Methods and Devices

A simple and improved method for diagnosis of tuberculosis using hypertonic saline and sodium hydroxide to concentrate and decontaminate sputum

Jessica N Ricaldi MD      Humberto Guerra MD PhD  

Instituto de Medicina Tropical Alexander von Humboldt, Av. Honorio Delgado 430, Lima 31, Republic of Peru

Correspondence to: Jessica Ricaldi, Instituto de Medicina Tropical Alexander von Humboldt, Av. Honorio Delgado 430, Lima 31, Republic of Peru Email: jricaldi{at}ucsd.edu

We report the use of hypertonic saline and sodium hydroxide (HS-SH) for concentration-decontamination of sputum samples for the diagnosis of tuberculosis. This method increases detection of mycobacteria in smears compared to examination of direct smears, is comparable and reduces contamination in cultures compared to NALC-NaOH.

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				C. A. Ganoza, J. N. Ricaldi, J. Chauca, G. Rojas, C. Munayco, J. Agapito, J. C. Palomino, and H. Guerra Novel hypertonic saline-sodium hydroxide (HS-SH) method for decontamination and concentration of sputum samples for Mycobacterium tuberculosis microscopy and culture J. Med. Microbiol., September 1, 2008; 57(9): 1094 - 1098. [Abstract] [Full Text] [PDF]